Document Type : Original Research Article

Authors

1 Department of Gynecology, General Hospital of Thessaloniki "St. Dimitrios" Thessaloniki, Hellas.

2 Department of Obstetrics & Gynecology, Aretaieion Hospital, Athens University, Athens, Attiki, Hellas.

3 Department of Surgery, Ippokrateion General Hospital, Athens University, Athens, Attiki, Hellas.

4 Department of Biologic Chemistry, Athens University, Athens, Attiki, Hellas.

5 Experimental Research Centre ELPEN Pharmaceuticals, S.A. Inc., Co., Pikermi, Attiki, Hellas.

6 6Experimental Research Centre ELPEN Pharmaceuticals, S.A. Inc., Co., Pikermi, Attiki, Hellas;

7 Experimental, Educational and Research Center ELPEN European University Cyprus, School of Medicine.

Abstract

Background and purpose:This study calculated the effects on serum chloride (Cl) levels, after treatment with either of 2 drugs: the erythropoietin (Epo) and the antioxidant lazaroid (L) drug U-74389G. The calculation was based on the results of 2 preliminary studies, each one of which estimated the certain influence, after the respective drug usage in induced ischemia-reperfusion (IR) animal experiment.
Materials and Methods: The 2 main experimental endpoints at which the serum Cl levels were evaluated was the 60th reperfusion min (for the groups A, C, and E) and the 120th reperfusion min (for the groups B, D, and F). Especially, the groups A and B were processed without drugs, groups C and D after Epo administration; whereas groups E and F after the L administration.
Results:The first preliminary study of Epo presented a non-significant hypochloremic effect by 0.74%+0.55% (p-value=0.1701). However, the second preliminary study of U-74389G showed a significant hypochloremic effect by 0.75%+0.34% (p-value=0.0310). These two studies were co-evaluated since they came from the same experimental setting. The outcome of the co-evaluation was that L is 1.012762-fold [1.011746 - 1.01378] more hypochloremic than Epo (p-value=0.0000).
Conclusion: The antioxidant capacities of U-74389G ascribe 1.012762-fold more hypochloremic effects than Epo (p-value=0.0000).

Graphical Abstract

Comparison of the Hypochloremic Effects of Erythropoietin and U-74389G

Keywords

Main Subjects

1. Τsompos C, Panoulis C, Τοutouzas K, Ζografos G, Papalois A. The effect of the antioxidant drug “U-74389G” on chloride during ischemia reperfusion injury in rats. Med Rev. 2014; 50(2): 40-44.         

2. Tsompos C, Panoulis C, Toutouzas K, Triantafyllou A, Zografos G, Papalois A. The effect of erythropoietin on chloride levels during hypoxia reoxygenation injury in rats. Signa Vitae. 2017; 13(2): 97-101. [crossref]

3. Román-Anguiano NG, Correa F, Cano-Martínez A, de la Peña-Díaz A, Zazueta C. Cardioprotective effects of Prolame and SNAP are related with nitric oxide production and with diminution of caspases and calpain-1 activities in reperfused rat hearts. Peer J. 2019; 29(7): 7348. [crossref]

4. Liu K, Wang F, Wang S, Li WN, Ye Q. Mangiferin attenuates myocardial ischemia-reperfusion injury via MAPK/Nrf-2/HO-1/NF-κB in vitro and in vivo. Oxid Med Cell Longev. 2019; 13: 7285434. [crossref]

5. Wang Y, Wu Z, Tian J. Intermedin protects HUVECs from ischemia reperfusion injury via Wnt/β-catenin signaling pathway. Ren Fail. 2019; 41(1): 159-166. [crossref]

6. Shu L, Zhang W, Huang G. Troxerutin attenuates myocardial cell apoptosis following myocardial ischemia-reperfusion injury through inhibition of miR-146a-5p expression. J Cell Physiol. 2019; 234(6): 9274-9282. [crossref]

7. Yin B, Hou XW, Lu ML. Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways. Acta Pharmacol Sin. 2019; 40(5): 599-607. [crossref]

8. Lou Z, Wang AP, Duan XM. Upregulation of NOX2 and NOX4 mediated by TGF-β signaling pathway exacerbates cerebral ischemia/reperfusion oxidative stress injury. Cell Physiol Biochem. 2018; 46(5): 2103-2113. [crossref]

9. Rao H, Bai Y, Li Q. SATB1 downregulation induced by oxidative stress participates in trophoblast invasion by regulating β-catenin. Biol Reprod. 2018; 98(6): 810-820. [crossref]

10. Mohamed AS, Hanafi NI, Sheikh Abdul Kadir SH. Ursodeoxycholic acid protects cardiomyocytes against cobalt chloride induced hypoxia by regulating transcriptional mediator of cells stress hypoxia inducible factor 1α and p53 protein. Cell Biochem Funct. 2017; 35(7): 453-463. [crossref]

11. Qiu LY, Duan GL, Yan YF. Sasanquasaponin induces increase of Cl /HCO3  exchange of anion exchanger 3 and promotes intracellular Cl  efflux in hypoxia/reoxygenation cardiomyocytes. Mol Med Rep. 2017; 16(3): 2953-2961. [crossref]

12. Zhang X, Du Q, Yang Y. The protective effect of Luteolin on myocardial ischemia/reperfusion (I/R) injury through TLR4/NF-κB/NLRP3 inflammasome pathway. Biomed Pharmacother. 2017; 91: 1042-1052.  [crossref]

13. Zhang XG, Zhao L, Zhang Y. Extracellular Cl--free-induced cardioprotection against hypoxia/reoxygenation is associated with attenuation of mitochondrial permeability transition pore. Biomed Pharmacother. 2017; 86: 637-644. [crossref]

14. Li YY, Xiao L, Qiu LY. Sasanquasaponin-induced cardioprotection involves inhibition of mPTP opening via attenuating intracellular chloride accumulation. Fitoterapia. 2017; 116: 1-9.  [crossref]

15. Xia Y, Liu Y, Xia T. Activation of volume-sensitive Cl- channel mediates autophagy-related cell death in myocardial ischaemia/reperfusion injury. Oncotarget. 2016; 7(26): 39345-39362.  [crossref]

16. Τsompos C, Panoulis C, Τοutouzas K. Comparison of the hyperkalemic effects of erythropoietin and U-74389G. Int J Womens Health Gynecol. 2019; 1(2): 107.

17. Tsompos C, Panoulis C, Toutouzas K. Comparison of the hyponatremic effects of erythropoietin and U-74389G. Insight Biomed Res. 2019; 3(1): 52-55.