Document Type : Original Research Article

Authors

Department of Oncology, Third Affiliated Hospital of Guizhou Medical University, Guizhou province, P.R. China.

Abstract

Background and purpose:To investigate the SPARC expression and relationship with survival in Colorectal cancer (CRC) patients in Han, Buyi and Miao race.
Methods: In our study, SPARC protein expression was detected in 90 available formalin-fixed paraffin-embedded (FFPE) tissues specimens from CRC patients in Han, Buyi and Miao race from the Third Affiliated Hospital of Guizhou Medical University by means of immunohistochemistry. According to the different expression of SPARC, the patients were divided into low expression group (SPARC score of 0+and 1+) and high expression group (SPARC score of 2+ and 3+). The correlations between the two groups in DFS and OS were analyzed, respectively.
Results:SPARC was mainly expressed in tumor cell cytoplasm and the positive rates of SPARC was 52.2% (47/90). There was no difference in the positive expression rate in the Han, Buyi and Miao race (P =0.87). SPARC expression was related to the pathological type (Adenocarcinoma). There was a marked associations in DFS between the low expression of SPARC group and high expression group (P=0.048, 0.047) in the Han and Miao ethnic. While the same result was no found in the Buyi race (P=0.176). There was no statistical significance in OS between the low expression of SPARC group and high expression group in the Buyi, Miao and Han race, respectively(P= 0.338, 0.424, 0.282).
Conclusion: SPARC was related with DFS in the Miao and Han race, while no correlation in the Buyi race.

Graphical Abstract

Study on the SPARC Expression and Relationship with Survival in Colorectal Cancer Patients in Han, Buyi and Miao Race

Keywords

[1].Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61(2): 69-90. [crossref]

[2].Brenner H, Kloor M, Pox CP. Colorectal cancer. Lancet. 2014; 383(9927): 1490-502. [crossref]

[3].Chiodoni C, Colombo MP, Sangaletti S. Matricellular proteins: from homeostasis to inflammation, cancer, and metastasis. Cancer Metastasis Rev. 2010; 29(2): 295-307. [crossref]

[4].Tai IT, Tang MJ. SPARC in cancer biology: its role in cancer progression and potential for therapy. Drug Resist Updat. 2008; 11(6): 231-246. [crossref]

[5].Takemasa I, Higuchi H, Yamamoto H, et al. Construction of preferential cDNA microarray specialized for human colorectal carcinoma: molecular sketch of colorectal cancer. Biochem Biophys Res Commun. 2001; 285(5): 1244-1249.

a.        [crossref]

[6].Chlenski A, Cohn SL. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21(1): 55-65. [crossref]

[7].Brabender J, Lord RV, Metzger R, Park J, Salonga D, Danenberg KD, Danenberg PV, Holscher AH, Schneider PM. Differential SPARC mRNA expression in Barrett’s oesophagus. Br J Cancer. 2003; 89(8): 1508-1512. [crossref]

[8].Wang CS, Lin KH, Chen SL, Chan YF, Hsueh S. Over expression of SPARC gene in human gastric carcinoma and its clinic-pathologic significance. Br J Cancer. 2004; 91(11): 1924-1930. [crossref]

[9].Alexandre C, Suan LC. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21(1): 55-65. [crossref]

[10].                     Jennbacken K, Vallbo C, Wang W, Damber JE. Expression of Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor-3 in human prostate cancer are associated with regional lymph node metastasis. Prostate. 2005; 65(2): 110116.

a.        [crossref]

[11].                     Serrero G, Ioffe O. Expression of the novel autocrine growth factor PC-Cell Derived Growth Factor in human breast cancer tissue. Hum Pathol. 2003; 34(11): 1148–1154. [crossref]

[12].                     Pan AP, Huang GY, Chen J. Relationship between hepatitis B virus covalently closed circular DNA and HBx protein expression in hepatocellular carcinoma and its significance. World Chin J Digestol. 2009; 17:712–715.

[13].                     Serrero G, Hawkins DM, Yue B, Ioffe O, Bejarano P, Phillips JT, Head JF, Elliott RL, Tkaczuk KR, Godwin AK, Weaver J, Kim WE. Progranulin (GP88) tumor tissue expression is associated with increased risk of recurrence in breast cancer patients diagnosed with estrogen receptor positive invasive ductal carcinoma. Breast Cancer Res. 2012; 14(1): R26. [crossref]

[14].                     Alexandre C, Susan LC. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21 (1): 55-65. [crossref]

[15].                     Yang E, Kang HJ, Koh KH, Rhee H, Kim NK, Kim H. Frequent inactivation of SPARC by promoter hypermethylation in colon cancers. Int J Cancer. 2007; 121(3): 567-575. [crossref]

 

[16].                     Liang JF, Wang HK, Xiao H, Li N, Cheng CX, Zhao YZ, Ma YB, Gao JZ, Bai RB, Zheng HX. Relationship and prognostic significance of SPARC and VEGF protein expression in colon cancer. J Exp Clin Cancer Res. 2010; 29: 71. [crossref]

1.       Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61(2): 69-90. [crossref]

2.       Brenner H, Kloor M, Pox CP. Colorectal cancer. Lancet. 2014; 383(9927): 1490-502. [crossref]

3.       Chiodoni C, Colombo MP, Sangaletti S. Matricellular proteins: from homeostasis to inflammation, cancer, and metastasis. Cancer Metastasis Rev. 2010; 29(2): 295-307. [crossref]

4.       Tai IT, Tang MJ. SPARC in cancer biology: its role in cancer progression and potential for therapy. Drug Resist Updat. 2008; 11(6): 231-246. [crossref]

5.       Takemasa I, Higuchi H, Yamamoto H, et al. Construction of preferential cDNA microarray specialized for human colorectal carcinoma: molecular sketch of colorectal cancer. Biochem Biophys Res Commun. 2001; 285(5): 1244-1249.

[crossref]

6.       Chlenski A, Cohn SL. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21(1): 55-65. [crossref]

7.       Brabender J, Lord RV, Metzger R, Park J, Salonga D, Danenberg KD, Danenberg PV, Holscher AH, Schneider PM. Differential SPARC mRNA expression in Barrett’s oesophagus. Br J Cancer. 2003; 89(8): 1508-1512. [crossref]

8.       Wang CS, Lin KH, Chen SL, Chan YF, Hsueh S. Over expression of SPARC gene in human gastric carcinoma and its clinic-pathologic significance. Br J Cancer. 2004; 91(11): 1924-1930. [crossref]

9.       Alexandre C, Suan LC. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21(1): 55-65. [crossref]

10.    Jennbacken K, Vallbo C, Wang W, Damber JE. Expression of Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor-3 in human prostate cancer are associated with regional lymph node metastasis. Prostate. 2005; 65(2): 110116.

[crossref]

11.    Serrero G, Ioffe O. Expression of the novel autocrine growth factor PC-Cell Derived Growth Factor in human breast cancer tissue. Hum Pathol. 2003; 34(11): 1148–1154. [crossref]

12.    Pan AP, Huang GY, Chen J. Relationship between hepatitis B virus covalently closed circular DNA and HBx protein expression in hepatocellular carcinoma and its significance. World Chin J Digestol. 2009; 17:712–715.

13.    Serrero G, Hawkins DM, Yue B, Ioffe O, Bejarano P, Phillips JT, Head JF, Elliott RL, Tkaczuk KR, Godwin AK, Weaver J, Kim WE. Progranulin (GP88) tumor tissue expression is associated with increased risk of recurrence in breast cancer patients diagnosed with estrogen receptor positive invasive ductal carcinoma. Breast Cancer Res. 2012; 14(1): R26. [crossref]

14.    Alexandre C, Susan LC. Modulation of matrix remodeling by SPARC in neoplastic progression. Semin Cell Dev Biol. 2010; 21 (1): 55-65. [crossref]

15.    Yang E, Kang HJ, Koh KH, Rhee H, Kim NK, Kim H. Frequent inactivation of SPARC by promoter hypermethylation in colon cancers. Int J Cancer. 2007; 121(3): 567-575. [crossref]

16.    Liang JF, Wang HK, Xiao H, Li N, Cheng CX, Zhao YZ, Ma YB, Gao JZ, Bai RB, Zheng HX. Relationship and prognostic significance of SPARC and VEGF protein expression in colon cancer. J Exp Clin Cancer Res. 2010; 29: 71. [crossref]